Journal: bioRxiv

Article Title: Diet-induced phospholipid remodeling dictates ferroptosis sensitivity and tumorigenesis in the pancreas

doi: 10.1101/2025.04.04.645324

Figure Lengend Snippet: (A) Disease burden (% disease/total pancreas area) for male KC mice fed control diet (CD) or designated HFDs for 12 weeks. Box plots designate 25 th , 50 th , and 75 th percentiles +/- min/max, n = 4-12 mice/diet. * p < 0.05, ** p < 0.01, Kruskal-Wallis test with Dunn’s post-hoc test. (B) Positive correlation between median disease burden of mice in (A) and dietary oleic acid concentration (µg/mg diet). Pearson correlation coefficient (R) and p -value are shown. (C) Stronger positive correlation between median disease burden of mice in (A) and mean total plasma oleic acid levels (nmol of FA/mL plasma) in non-tumor-bearing C57BL/6 male mice fed each diet for 12 weeks (n = 5 mice/diet). Pearson correlation coefficient (R) and p -value are shown. (D) Representative H&E and Ck19 (duct tumor marker) immunostaining of pancreata of male KC mice fed CD, LODs, or HODs for 12 weeks. Scale bars are 50 µm. (E) Disease burden (mean +/- s.e.m., n = 7-12 mice/diet) for male KC mice fed CD, LODs, or HODs for 12 weeks. * p < 0.05, ** p < 0.01, Kruskal-Wallis test with Dunn’s post-hoc test. (F) Proportional area (mean percentage +/- s.e.m., n = 6-9 mice/diet) of total disease by lesion type (ADM, PanIN, PDAC) of KC mice fed CD, LODs, or HODs. Proportions are not significantly different across diets, Kruskal-Wallis test with Dunn’s post-hoc test.

Article Snippet: The following primary antibodies were used: mouse anti-Ck19 (DSHB TROMA-III, 1:500), rabbit anti-Ki67 (Biocare Medical CRM325, 1:200), rat anti-p19 ARF (Santa Cruz Biotech sc-32748, 1:100), rat anti-Cd45 (Abcam ab25386, 1:100), rabbit anti-F4/80 (CST 70076, 1:400), rabbit anti-SMA (Invitrogen PA1-37024, 1:100), mouse anti-B220 (BD Pharmingen 550286, 1:40), rabbit anti-Cd3 (CST 78588, 1:200), rabbit anti-4-HNE (Abcam, ab46545, 1:200), and rabbit anti-Nqo1 (Sigma-Aldrich HPA007308, 1:100).

Techniques: Control, Concentration Assay, Marker, Immunostaining

Journal: JAMA Facial Plastic Surgery

Article Title: Use of Condensed Nanofat Combined With Fat Grafts to Treat Atrophic Scars

doi: 10.1001/jamafacial.2017.1329

Figure Lengend Snippet: A and B, Under Fontana-Masson staining, an increase in melanin is seen in the basal cell layer between the preoperative (A) and 6-month postoperative (B) specimens (original magnification ×400). C and D, Under cytokeratin (CK) 14 staining, almost no sebaceous or sweat glands were observed preoperatively (C), but they were clearly visualized 6 months postoperatively (D) (original magnification ×200). E and F, Under CK19 staining, almost no sebaceous or sweat glands were observed preoperatively (E), but they were clearly visualized 6 months postoperatively (F) (original magnification ×100).

Article Snippet: Following citrate incubation and blocking with 5% rabbit serum for 30 minutes, slides were incubated at 4°C overnight with the following antibodies against cytokeratin (CK) 14 and CK19 (both at 1:200 concentration; ProteinTech).

Techniques: Staining

Journal: STAR Protocols

Article Title: Determination of the FABP5 expression profile in skin lesions of an IMQ-induced psoriasis mouse model using flow cytometry

doi: 10.1016/j.xpro.2024.103018

Figure Lengend Snippet: Keratinocyte panel

Article Snippet: Rabbit polyclonal anti-mouse cytokeratin 6A (1:300) , Proteintech , Cat#10590-1-AP, RRID: AB_2134306.

Techniques: Concentration Assay, Staining

Journal: STAR Protocols

Article Title: Determination of the FABP5 expression profile in skin lesions of an IMQ-induced psoriasis mouse model using flow cytometry

doi: 10.1016/j.xpro.2024.103018

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-mouse cytokeratin 6A (1:300) , Proteintech , Cat#10590-1-AP, RRID: AB_2134306.

Techniques: Recombinant, Staining, Cream, Software, Flow Cytometry

Journal: Journal of Translational Medicine

Article Title: Lysine acetyltransferase 14 mediates TGF-β-induced fibrosis in ovarian endometrioma via co-operation with serum response factor

doi: 10.1186/s12967-024-05243-2

Figure Lengend Snippet: KAT14 overexpression exacerbated TGF-β1-induced fibrogenesis in immortalized HESCs. ( A) Bar graph showing the enrichment analysis of upregulated genes after KAT14 overexpression in terms of GO molecular function. ( B) Gene set enrichment analysis (GSEA) of DEGs induced by KAT14 overexpression compared to the negative control showing significant enrichment in gene sets associated with extracellular matrix structural constituents and ECM component pathways. Normalized enrichment score (NES), false discovery rate (FDR), and P-value s are shown. ( C) Heatmap profile of DEGs associated with fibroblast activation and ECM remodeling in KAT14-overexpressed cells compared to control cells based on RNA-Seq ( n = 3). ( D) qRT-PCR analysis of the relative mRNA expression of FN1 , COL1A1 , and ACTA2 in HESCs infected with the indicated lentiviruses harboring KAT14 expression vector (pCDH- KAT14 ) or empty vector control (pCDH-Ctrl) treated with TGF-β1 for 24 h. Relative quantification of gene expression was calculated using the 2 −∆∆Ct method and normalized to GAPDH as the internal control. ( E) Western blots measuring the protein level of fibronectin, collagen I, and α-SMA in HESCs infected with pCDH- KAT14 or pCDH-Ctrl lentiviruses under TGF-β1 stimulation for 48 h. ( F) Immunofluorescence staining showing the expression of KAT14, α-SMA, and the ECM molecules fibronectin and collagen I in HESCs infected with pCDH- KAT14 or pCDH-Ctrl lentiviruses under TGF-β1 stimulation for 48 h. Scale bar: 50 μm. ( G) Collagen gel contractility assay showing the cell contraction capacity of HESCs infected with pCDH- KAT14 or pCDH-Ctrl lentiviruses under TGF-β1 stimulation for 24 h. The degree of collagen gel contraction was determined as the difference between the diameters of the well and the released gels. ( H) Wound healing assay showing the migration ability of HESCs infected with lentiviral vector containing pCDH- KAT14 or pCDH-Ctrl treated with TGF-β1 for 0, 18, and 36 h. Wound healing was assessed by calculating the area in µm 2 between the lesion edges. Scale bar: 200 μm. TGF-β1 was used at a concentration of 12 ng/ml. Data are representative of three or more independent experimental replicates. For all panels, data are presented as the mean ± SD. P -values were determined by Student’s t-test in panels ( D , E , G , H ). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. HESC: human endometrial stromal cell, GO: gene ontology, DEGs: differentially expressed genes, Ctrl: control

Article Snippet: The immortalized human endometrial stromal cell line, generously provided by Prof. Haibin Wang (Xiamen University), underwent cell immunofluorescence staining to determine the purity of the human endometrial stromal cells (HESCs) using pan-cytokeratin (#26411-1-AP, Proteintech, RRID: AB_2880505), CD10 (#23898-1-AP, Proteintech, RRID: AB_2879354), and vimentin (#60330-1-Ig, Proteintech, RRID: AB_2881439) (Additional file 1: Fig. A), as previously described [ ].

Techniques: Over Expression, Negative Control, Activation Assay, Control, RNA Sequencing, Quantitative RT-PCR, Expressing, Infection, Plasmid Preparation, Quantitative Proteomics, Gene Expression, Western Blot, Immunofluorescence, Staining, Wound Healing Assay, Migration, Concentration Assay